Description
It’s Purpose:
New Brunswick Scientific G-25 is a type of gel filtration resin used for protein purification. It consists of spherical beads that separate molecules based on their size by allowing smaller molecules to enter the pores within the beads, while larger molecules are excluded and eluted earlier. It has a molecular weight exclusion limit of approximately 25,000 Daltons, which makes it suitable for separating smaller proteins and peptides
Acme’s Summary:
New Brunswick Scientific G-25 is used for protein purification because it is a type of gel filtration resin that separates molecules based on their size. Gel filtration is a common method for purifying proteins and separating them from other biomolecules, such as nucleic acids, lipids, and carbohydrates. It is also useful for desalting protein samples and exchanging buffers. G-25 has a molecular weight exclusion limit of approximately 25,000 Daltons, which makes it suitable for separating smaller proteins and peptides. This resin is available in pre-packed columns, which simplifies the purification process and reduces the risk of contamination.
Top 3 Competitors
- Sephadex G-25: Sephadex is a brand of gel filtration resins made by GE Healthcare. Sephadex G-25 is a similar resin to New Brunswick Scientific G-25, with a similar exclusion limit of approximately 5-30 kDa.
- Bio-Gel P-30: Bio-Gel is a brand of gel filtration resins made by Bio-Rad Laboratories. Bio-Gel P-30 is a resin that has a similar exclusion limit to New Brunswick Scientific G-25 of approximately 5-30 kDa.
- Superdex 75: Superdex is a brand of gel filtration resins made by GE Healthcare. Superdex 75 is a higher resolution resin that can separate molecules based on a wider range of molecular weights (3-70 kDa), making it useful for more complex protein mixtures.
5 Best Things:
- Gel filtration resin: New Brunswick Scientific G-25 is a type of gel filtration resin used for protein purification. It is made of spherical beads that separate molecules based on their size by allowing smaller molecules to enter the pores within the beads.
- Molecular weight exclusion limit: G-25 has a molecular weight exclusion limit of approximately 25,000 Daltons, which makes it suitable for separating smaller proteins and peptides.
- Pre-packed columns: G-25 resin is available in pre-packed columns, which simplifies the purification process and reduces the risk of contamination.
- High purity: G-25 resin is highly purified to minimize background interference, ensuring high-quality results.
- Compatibility with common buffers: G-25 resin is compatible with a wide range of common buffers and can be used for buffer exchange and desalting of protein samples.